PAT Roberts

Longwave pass filters and bio-imaging

Longwave pass filters block short wavelengths of light, allowing for their use in optical filters near infrared wavelengths.

With new work on bio-imaging techniques, this could lead to better views of tumour cells, or of the response of the whole body to certain drugs.

A team at the German Research Centre for Environmental Health (Helmholtz Zentrum Munchen) have reported on one method of bio-imaging using fluorescent markers.

Their new markers, created by transferring genetic information from the protein eqFP615 into the DT40 chicken B cell line, emit in the infrared spectrum.

As such, optical filters near infrared can allow their fluorescence to be viewed without interference from other wavelengths.

By using longwave pass filters in this way, better bio-imaging should be a reality in the years to come.

The markers depend on triggering an evolutionary mechanism by introducing genetic material into B cells from the immune system, creating new genetic and protein variants in the process.

Research team member Randolph Caldwell said: “We have demonstrated the further use of this novel technology to develop highly sought-after biologically relevant fluorescent markers quickly and easily for different imaging needs.”